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, 1995), physiological measures such as the facial electromyograph (EMG; eg, Cacioppo & Petty, 1979a) or electroencephalogram (EEG; eg, Cacioppo, Crites, Bernston, & Coles, 1993), and physical behaviors like nonverbal gestures, eye contact, or seating distance (eg, Argyle & Dean, 1965; Dovidio, Kawakami, Johnson, Johnson, & Howard, 1997; Macrae, Bodenhausen, Milne, & Jetten, 1994; Word, Zanna, & Cooper, 1974) Direct and indirect measurement methods typically exhibit modest positive correlations (Dovidio, Kawakami, & Beach, 2000) It is important to note that direct and indirect measurement methods can differ in the extent to which they permit deliberative responding (Vargas, von Hippel, & Petty, 2001) For example, experimenters could require individuals to report their attitudes on a direct one-item rating scale very quickly with no time for deliberation, or they could permit individuals to make the judgment after some minimal or extensive re ection.

are in parallel (note: the VDD and VSS are both at ground potential during a.c. analysis), where one element is in a forward bias d.c. voltage and the other element is in reverse bias. In the RF ESD design of ESD input networks, the following RF ESD design practices can be applied: Linearity improvement by utilization of a reverse-bias and forward-bias element in a common circuit to compensate the voltage dependence of a single element. Linearity improvement by utilization of different sizes of reverse and forward bias elements (e.g., using the same element). Linearity improvement by utilization of different types of reverse and forward bias elements. Utilization of the same element for the up and down diode to provide independence from the substrate wafer. Utilization of the same element for the up and down diode for an improved power-tofailure symmetry. Utilization of the same element for the up and down diode for an improved tracking and matching.

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bone [Dennis and Caplan, 1993; Lennon et al, 2001] On the other hand, cartilage is more commonly observed than bone for rabbit MSCs The in vivo ceramic cube assay system is complemented by the use of a number of in vitro assays, each of which is speci c for different differentiated cells Elevated alkaline phosphatase activity, although not unique to osteogenic cells, is a useful early marker for osteogenic differentiation Accordingly, biochemical and cytochemical assays for alkaline phosphatase are used early in the culture of MSCs in osteoinductive conditions On the other hand, calcium biochemistry and von Kossa staining to detect mineralization are used later in the culture period These assays and the conditions that promote osteogenic differentiation are described in Section 7 Aggregate or pellet cultures can be established in a de ned medium to promote chondrogenic differentiation of MSCs [Johnstone et al, 1998].

200% 180 Lymphoid Size attained in % of 20-year-old size 160 140 120 100 Brain and head 80 60 40 20 0 0 2 General Reproductive 4 6 8 10 12 14 16 18 20 Age (years)

Similarly, some indirect attitude measures permit relatively slow.

Such cultures will not be covered in this section as they are treated thoroughly in 4 of this book Differentiation of MSCs to adipocytes can be induced through the use of a unique medium in cultures seeded at a slightly higher density than that used for osteogenic differentiation Flow cytometric analysis after Nile Red staining is used to assess expression of the adipocytic phenotype after induction This methodology is described in Section 8 The ability of MSCs to differentiate along these various phenotypic lines is strongly suggestive of their stem cell nature MSCs, although sometimes referred to with different terminology and derived from a number of species, have also been shown to differentiate into other mesenchymal lineages, including skeletal muscle [Wakitani et al, 1995], cardiac muscle [Tomita et al, 1999], and hematopoietic supportive tissue [Ko et al.

follows the rest of the emotional train Apart from the theological implications for the a priori ability to make judgments of good and bad, psychological theory in the twentieth century places more emphasis on the conditions and processes that give rise to such judgments The American attack on James came primarily from E B Titchener, who also started with fundamental feelings, though in a more complex form and with a somewhat less unanalyzable quality The feeling is in reality a complex process, composed of a perception or idea and affection, in which affection plays the principal part (Titchener, 1896, p 214).

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